GH axis research
Studies of GRF receptor signalling using the modified 1-29 sequence rather than the native peptide.
Peptide · Growth hormone axis
CJC-1295 without DAC
The unmodified half of a confusing pair. Same GRF backbone as the DAC product, without the linker — which changes the mass, the counter-ion and the handling.
Also known as CJC-1295 no DAC · Mod GRF 1-29 · modified GRF(1-29) · tetrasubstituted GRF
Sequence · 29 residues
AcidicBasicHydrophobicPolar
- Residues
- 29
- Molecular weight
- 3367.9
- Formula
- C152H252N44O42
- Purity
- 98.7% HPLC
Identity data as printed on the batch certificate of analysis.
Overview
What CJC-1295 without DAC is
CJC-1295 without DAC is a 29-residue modified GRF(1-29): four substitutions against the native sequence, a D-alanine at position 2, and a C-terminal amide. Molecular weight 3,367.9, CAS 863288-34-0, supplied as the acetate.
The name is the problem. “CJC-1295” is sold both with and without a drug-affinity complex — a maleimidopropionic acid linker that lets the peptide associate with albumin. The two are different molecules: the DAC form is heavier, is supplied as the trifluoroacetate, and behaves differently in solution. Our catalogue carries both, as ES005 (this page) and ED0003, with separate certificates that do not transfer between them.
The lot on file is from August 2025, within its retest period to August 2027. If you need a fresher batch for a bulk order, say so with the quantity — a newer lot can be quoted rather than shipped from this one.
- 29-residue modified GRF(1-29), C-terminal amide, 3,367.9 g/mol
- 98.7% by HPLC; total impurities 1.3%, largest single 0.5%
- Acetate 6.1%, peptide content 86.8% — both stated separately
- Distinct from the DAC form (ED0003): different mass, different counter-ion
Identity
The record.
The identifiers your specification sheet, customs paperwork and product label all have to agree on. Where a number depends on the salt form, both are given.
- Chemical name
- Modified GRF(1-29), C-terminal amide, acetate salt
- Common name
- CJC-1295 without DAC; Mod GRF 1-29
- CAS number
- 863288-34-0
- Molecular formula
- C₁₅₂H₂₅₂N₄₄O₄₂
- Molecular weight
- 3367.9 g/mol
- Residue count
- 29 (C-terminal amide, D-Ala at position 2)
- Counter-ion
- Acetate — 6.1% on the current lot
- Appearance
- White or almost white powder
- Solubility
- Soluble in water
- Water content
- ≤ 8.0% (Karl Fischer)
- Peptide content
- 86.8% (nitrogen determination)
- Storage
- −20 ± 5 °C, sealed, protected from light
HS codes are indicative only. Final classification is confirmed per shipment with your customs broker.
Manufacture
How it is made.
The four substitutions are deliberate — they are what makes this a modified GRF rather than the native sequence.
- 01
Solid-phase assembly
The 29-residue chain is built stepwise, with D-alanine introduced at position 2. A D residue at that position is not incidental: it changes how the peptide is recognised and how it resists degradation.
- 02
C-terminal amidation
The amide is part of the molecule and is confirmed by mass spectrometry. The free acid would be 1 Da lighter and a different product.
- 03
Purification
Gradient HPLC at 220 nm against the full-length sequence. Total impurities on the current lot are 1.3%, with the largest single at 0.5%.
- 04
Acetate exchange and drying
Supplied as the acetate, at 6.1% on this lot — roughly a third of the acetate load typical of the DAC form, which is supplied as the trifluoroacetate.
Applications
Where it is used.
Supplied to laboratories working with modified GRF analogues. Research use only.
Comparative work
Direct comparison against the DAC form, where the absence of the linker is the variable under study.
Analytical development
A 3,368 Da amide-terminated peptide that makes a workable reference standard for methods targeting the GRF family.
Specification
Release criteria.
The written limits we release against, with the method used to test each one. Batch results for the lot you receive are stated on its certificate of analysis.
| Parameter | Limit | Method |
|---|---|---|
| Appearance | White or almost white powder | Visual |
| Solubility | Soluble in water | Visual |
| Identification by MS | 3367.90 ± 1 | ESI-MS |
| Water content | ≤ 8.0% | Karl Fischer |
| Acetic acid | ≤ 15.0% | HPLC |
| Purity | ≥ 98.0% | HPLC |
| Peptide content | ≥ 85.0% | Nitrogen determination |
| Total impurities | ≤ 2.0% | HPLC |
| Largest single impurity | ≤ 1.0% | HPLC |
| Acetonitrile residue | ≤ 0.041% | GC |
| Dichloromethane residue | ≤ 0.060% | GC |
| DMF residue | ≤ 0.088% | GC |
| Bacterial endotoxin | < 10 EU/mg | LAL |
| Total aerobic count | ≤ 100 cfu/g | Plate count |
| Mould & yeast | ≤ 100 cfu/g | Plate count |
Tighter limits — for example a lower heavy-metal ceiling or a residual-solvent panel — can be quoted against your own specification wording.
HPLC · ES005
Certificate
Reading the certificate.
The first thing to check on a CJC certificate is which of the two molecules it describes.
Mass first, name second
This product is 3,367.9 g/mol. The DAC form is heavier and is supplied as the trifluoroacetate. If the certificate shows a TFA figure in the teens, you are looking at the DAC product's documentation, not this one.
Counter-ion tells you the salt, and the salt matters
Acetate at 6.1% here; the DAC form runs a much higher trifluoroacetate load. Two powders that look identical in a photograph behave differently in an assay.
Peptide content 86.8%, not 98.7%
Purity is the chromatographic figure; peptide content is how much of the powder is peptide. With 6.1% acetate and 4.9% water, a 10 mg weighing holds about 8.7 mg of peptide.
Documentation & packaging
What ships with it.
The paperwork travels with the goods. If your QA team needs an extra declaration, ask before the order is released — some are issued per production lot and cannot be retrofitted.
Certificate of analysis
Purity, impurities, acetate, water, peptide content, identity by MS and microbiological results.
Analytical data
HPLC chromatogram at 220 nm (Kromasil 100-5-C18, 30→55% over 25 min) and the ESI mass result.
SDS & specification
Safety data sheet and the enterprise standard the batch is released against.
Batch documentation
Lot number, manufacture date and retest date. The data sheet covers this product only.
| Pack size | Presentation | Typical use |
|---|---|---|
| 1–10 g | Amber vial, 10 mL | Evaluation |
| 25–50 g | Amber vial, 50 mL | Pilot scale |
| 100 g | Amber vial, 100 mL | Small commercial |
| 1 kg+ | Foil bag, quoted per order | Bulk |
Questions
Before you order.
The questions buyers actually ask about CJC-1295 (without DAC). Anything not covered here, ask directly — you will get an answer from someone technical, not a template.
What is the difference from CJC-1295 with DAC?
The DAC form carries a maleimidopropionic acid linker that lets the peptide associate with albumin. That changes the molecular weight, the counter-ion (trifluoroacetate rather than acetate) and the behaviour in solution. They are two products, with two certificates, and one cannot substitute for the other.
Which catalogue code is which?
ES005 is this product, CJC-1295 without DAC. ED0003 is CJC-1295 with DAC. Quote the code on the purchase order — the trade name alone is ambiguous.
How old is the batch on file?
Manufactured August 2025, within its retest period to August 2027. For a bulk order, ask for the newest batch available rather than shipping from this one.
Why is peptide content 86.8% when purity is 98.7%?
Because the powder contains acetate and water as well as peptide. Purity describes the peptide fraction; peptide content describes its share of the total mass. Weigh against 86.8%.
Is the C-terminus amidated?
Yes. The amide is introduced at cleavage and confirmed by mass spectrometry against 3,367.90. The free-acid form would be a different molecule.
How should it be stored?
−20 ± 5 °C, sealed and protected from light. Equilibrate to room temperature before opening.
Related
Often quoted together.
Quotation
Ask for CJC-1295 (without DAC), by the gram.
Send the quantity, the grade you need and the destination. You will get a written quotation with lead time, packaging options and the documentation set for this material.